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Image Search Results
Journal: Cell Death & Disease
Article Title: Downregulation of Engulfment and cell motility 1 (Elmo1) induces quiescence and resistance to poly(I:C)-induced apoptosis in endothelial cells
doi: 10.1038/s41419-025-08341-1
Figure Lengend Snippet: a Transcript levels of selected genes associated with apoptosis before (0 h) and after (24 h) the poly(I:C) treatment. Transcript levels of each gene are shown as TPM (transcript per million). Data are mean ± SE ( N = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, n.s . not significant. b Representative images of Mock, siNT and siELMO1 cells 24 h after the poly(I:C) stimulation. Cells were treated with or without 20 ng/ml of caspase-8 inhibitor Z-IETD-FMK throughout the experiment. Bar = 20 μm. c Cell death in (b) was quantified as in Fig. . Data are mean ± SD ( N = 3). *** p < 0.001. d Western blot of full-length and cleaved caspase-8, -3 and -7 before (0 h) and after (4 h, 8 h) the stimulation with poly(I:C). Beta-Actin was detected as a loading control. Images are representative of three independent experiments.
Article Snippet: The absence of mycoplasma contamination was confirmed using the Mycoplasma PCR Detection Kit (Abcam, Cambridge, MA, USA),
Techniques: Western Blot, Control